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Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma

Yıl 2011, Cilt: 6 Sayı: 3, 183 - 193, 21.12.2011

Öz

Bu araştırmada, yetişkin bıldırcınların gastrointestinal sistemindeki gastrin (G hücreleri), somatostatin (D hücreleri) ve
serotonin (EC hücreleri) immunopozitif hücrelerin dağılımı, yerleşimi ve sıklığının immunohistokimyasal yöntemlerle
belirlenmesi amaçlandı. Çalışmada, 10 adet sağlıklı yetişkin bıldırcının glanduler ve musküler mideleri, duodenum, jejunum
ve ileumları materyal olarak kullanıldı. Alınan doku kesitleri streptavidin biotin peroksidaz yöntemi ile immunohistokimyasal
olarak boyandı. Gastrin ve serotonin immunopozitif hücrelerin glanduler ve musküler midelere göre ince barsaklarda daha
fazla olduğu belirlendi. Bununla birlikte, somatostatin immunopozitif hücreler mide ve ince barsakların tamamında çok az
sayıda görüldü. Ayrıca, G, D ve EC hücrelerinin her iki midede genellikle kapalı tip, ince barsak bölümlerinde ise hem açık
hem de kapalı tip endokrin hücreler halinde bulunduğu da tespit edildi. Sonuç olarak, bıldırcınların glanduler ve musküler
mideleri, duodenum, jejunum ve ileumlarında G, D ve EC hücrelerinin değişen sayılarda bulunduğu belirlendi. Ayrıca,
duodenum ve ileum mukozasında, G ve EC hücrelerinin D hücrelerine göre daha fazla bulunduğu da tespit edildi.

Kaynakça

  • Aluments J., Sundler F., Hakånson R., 1977. Distribution, ontogeny and ultrastructure of somatostatin immunreactive cells in the pancreas and gut. Cell Tissue Res., 185, 465– 479.
  • Bezuidenhout AJ., Van Aswegen G., 1990. A light microscopic and immunocytochemical study of the gastrointestinal tract of the ostrich (Struthio camelusL.). Onderstepoort J. Vet. Res., 57, 37-48.
  • Bordi C., D’adda T., Azzoni C., Ferraro G., 2000. Classification of gastric endocrine cells at the light and electron microscopical levels. Micros. Res. Tech. 48, 258–271.
  • Bueno L., De Ponti F., Fried M., Kullak-Ublick GA., Kwiatek MA., Pohl D., Quigley EM., Tack J., Talley NJ., 2007. Serotonergic and non-serotonergic targets in the pharmacotherapy of visceral hypersensitivity. Neurogastroenterol. Motil., 19 (Suppl. 1), 89-119.
  • Castaldo L., Lucini C., 1991. An immunohistochemical study on the endocrine cells in the gastrointestinal tract of domestic duck. Eur. J. Bas. Appl. Histo-chem., 35, 131-143.
  • Coleman S., Foley S., Dunlop SP., Wheatcroft J., Blackshaw E., Perkins AC., Singh G., Marsden CA., Holmes GK., Spiller RC., 2006. Abnormalities of serotonin metabolism and their relation to symptoms in untreated celiac disease. Clin. Gastroenterol. Hepatol., 4, 874– 881.
  • Cetin Y., Kuhn M., Kulaksız H., Adermann K., Bargsten G., Grube D., Forssmann WG., 1994. Enterochromaffin cells of the digestive system: Cellular source of guanylin, a guanylate cyclase-activating peptide. Proc. Nati. Acad. Sci. USA., 91, 2935-2939.
  • Çınar K., Diler D., 2008. Localizations of cells that secrete bombesin, glucagon, somatostatin-14 in stomach (proventriculus and gizzard) in chicken (Gallus gallus domestica L.) during the prenatal and postnatal periods. Ankara Üniv. Vet. Fak. Derg., 55, 201-206.
  • Gençer Tarakçı B., Yaman M., Bayrakdar A., Yaman I., 2008. Immunohistochemical study on the endocrine cellsin the gastrointestinal tract of the ostrich. Medycyna Wet., 64, 64-67
  • Dall’Aglio C., Scocco P., Ceccarelli P., Pedini V., 1998. Neuroendocrine cells in the gastro-intestinal tract of the wild boar. Anat. Histol. Embryol., 27, 381-385.
  • Gershon MD., Tack J., 2007. The serotonin signaling system: From basic understanding to drug development for functional GI disorders. Gastroenterology, 132, 397–414.
  • Girgin A., Alabay B., Liman N., Özfiliz N., Gülmez N., Özcan Z., Yörük M., Erdost H., Aslan Ş., Ergün L., Zık. B., 2009. Veteriner Özel Histoloji, Editör, Özer A. 1. Baskı. Sayfa 73-76, 167-168
  • Gulmez N., Nazli M., Aslan S., Liman N., 2003. Immunolocalisation of serotonin, gastrin, somatostatin and glucagon in entero-endocrine cells of the goose (Anser anser). Acta Vet. Hung., 51, 439-449.
  • Kobayashi S., Fujita T., Sasagawa T., 1971. Electron-microscope studies on the endocrine cells of the humangastric fundus. Arch. Histol. Jap., 32, 429-444.
  • Ku SK., Lee JH., Lee HS., 1998. The histological and immunohistological studies in the Meckel's diverticulum of the duck, Anas platyrhynchos platyrhyncos, Linne. Korean J. Vet. Res., 38, 450-454.
  • Ku SK., Lee HS., Park KD., Lee JH., 2000. Immuno histochemistry of gastrointestinal endocrine cells in the Meckel's diverticulum of the Bean goose, Anser fabalis latham. Korean J. Biol. Sc i., 4, 375 -379.
  • Martinez A., López J., Sesma P., 1993. Development of the diffuse endocrine system in the chicken proventriculus. Cell Tissue Res., 271, 107-113.
  • Naruse H., Gomi T., Kimura A., Adriaensen D., Timmermans JP., 2005. Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells. Anat. Sci. Int., 80, 97-104.
  • Okamoto T., Yamada Y., Iwanaga T., 1980. Distribution and ultrastructure of gastrin cells in the duck digestive tract. Nippon Juigaku Zasshi. 42, 643-649.
  • Oomori Y., Okada Y., Watanabe T., 1997a. Immunohistochemical localization of serotonin, galanin, cholecystokinin and methionine-enkephalin in adrenal medullary cells of the chicken. Tissue Cell, 29, 199-205.
  • Oomori Y., Satoh Y., Ishikawa K., Gesase AP., 1997b. Exocytosis in the antral gastrin cells of mouse, rat, and guinea pig after stimulation by carbamylcholine. Cell Tissue Res., 289, 463–472.
  • Park KD., Lee JH., Ku SK., Lee HS., 1999. An immunohistochemical study on the gastrointestinal endocrine cells in the bean goose, Anser fabalis Latham. Korean J. Vet. Res. 39, 1038-1048.
  • Rawdon BB., 2001. Morphogenesis of gut and distribution of the progenitors of gut endocrine cells at cranial somite levels of the chick embryo. Develop. Dynamics, 222, 153–164.
  • Rawdon BB., Andrew A., 1994. Distribution of serotonin-immunoreactive gut endocrine cells in chicks at hatching. Histochemistry, 102, 93-100.
  • Saito T., Yamada J., Kitamura N., Yamashita T., 1989. An immunohistochemical study on the distribution of endocrine cells in the gastrointestinal tract of domestic pigeon, (Columba livia var domestica). Z. Mikrosk. Anat. Forsc., 103, 237-246.
  • Salvi E., Renda T., 1986. Immunohistochemical studies on the ontogenesis of some endocrine cells in the chicken antrum and duodenum. Basic Appl. Histochem., 30, 307-316.
  • Sikander A., Rana SV., Prasad KK., 2009. Role of serotonin in gastrointestinal motility and irritable bowel syndrome. Clinica Chimica Acta, 403, 47–55.
  • Solcia E., Rindi G., Buffa R., Fiocca R., Capella C., 2000. Gastric endocrine cells: types, function and growth. Regul. Pept., 93, 31-35.
  • Sundler F., Håkanson R., 1991. Gastric endocrine cell typing at the light microscopic level. In: Håkanson R, Sundler F, editor. The stomach as an endocrine organ. Amsterdam: Elsevier, p 9–26
  • Şimşek N., Alabay B., 2008. Light and electron microscopic examinations of the pancreas in quails (Coturnix coturnix japonica). Revue Med. Vet., 159, 198–206.
  • Watanabe T., Chikazawa H., Chungsamarnyart N., Fujioka T., Yamada Y., 1987. Serotonin-storing cells of the chicken duodenum: light, fluorescence, electron microscopy and immunohistochemistry. Cell Tissue Res., 247, 25-32.
  • Wu SV., Wang YH., Campbell BJ., Dimaline R., 1992. Avian progastrin: structure, antral gene expression and biological functions during achlorhydria. Regul. Pept., 40, 283.
  • Yamada J., Sugitani A., Yamashita T., Misu M., 1980. Exocytotic granule release in quail gastrin cells. Biomed. Res., 1, 24-27.
  • Yamaguchi S., Yamada J., Kitamura N., Yamashita T., 1986. Ontogeny of the endocrine cells in the quail proventriculus. Z. Mikrosk. Anat. Forsc., 100, 981-989.
  • Yamaguchi S., Yamada J., Kitamura N., Yamashita T., 1987. Histological and immunohistochemical study on ontogeny of the endocrine cells in the quail gizzard. Gegenbaurs Morpho. Jahrb., 133, 71-78.
Yıl 2011, Cilt: 6 Sayı: 3, 183 - 193, 21.12.2011

Öz

Kaynakça

  • Aluments J., Sundler F., Hakånson R., 1977. Distribution, ontogeny and ultrastructure of somatostatin immunreactive cells in the pancreas and gut. Cell Tissue Res., 185, 465– 479.
  • Bezuidenhout AJ., Van Aswegen G., 1990. A light microscopic and immunocytochemical study of the gastrointestinal tract of the ostrich (Struthio camelusL.). Onderstepoort J. Vet. Res., 57, 37-48.
  • Bordi C., D’adda T., Azzoni C., Ferraro G., 2000. Classification of gastric endocrine cells at the light and electron microscopical levels. Micros. Res. Tech. 48, 258–271.
  • Bueno L., De Ponti F., Fried M., Kullak-Ublick GA., Kwiatek MA., Pohl D., Quigley EM., Tack J., Talley NJ., 2007. Serotonergic and non-serotonergic targets in the pharmacotherapy of visceral hypersensitivity. Neurogastroenterol. Motil., 19 (Suppl. 1), 89-119.
  • Castaldo L., Lucini C., 1991. An immunohistochemical study on the endocrine cells in the gastrointestinal tract of domestic duck. Eur. J. Bas. Appl. Histo-chem., 35, 131-143.
  • Coleman S., Foley S., Dunlop SP., Wheatcroft J., Blackshaw E., Perkins AC., Singh G., Marsden CA., Holmes GK., Spiller RC., 2006. Abnormalities of serotonin metabolism and their relation to symptoms in untreated celiac disease. Clin. Gastroenterol. Hepatol., 4, 874– 881.
  • Cetin Y., Kuhn M., Kulaksız H., Adermann K., Bargsten G., Grube D., Forssmann WG., 1994. Enterochromaffin cells of the digestive system: Cellular source of guanylin, a guanylate cyclase-activating peptide. Proc. Nati. Acad. Sci. USA., 91, 2935-2939.
  • Çınar K., Diler D., 2008. Localizations of cells that secrete bombesin, glucagon, somatostatin-14 in stomach (proventriculus and gizzard) in chicken (Gallus gallus domestica L.) during the prenatal and postnatal periods. Ankara Üniv. Vet. Fak. Derg., 55, 201-206.
  • Gençer Tarakçı B., Yaman M., Bayrakdar A., Yaman I., 2008. Immunohistochemical study on the endocrine cellsin the gastrointestinal tract of the ostrich. Medycyna Wet., 64, 64-67
  • Dall’Aglio C., Scocco P., Ceccarelli P., Pedini V., 1998. Neuroendocrine cells in the gastro-intestinal tract of the wild boar. Anat. Histol. Embryol., 27, 381-385.
  • Gershon MD., Tack J., 2007. The serotonin signaling system: From basic understanding to drug development for functional GI disorders. Gastroenterology, 132, 397–414.
  • Girgin A., Alabay B., Liman N., Özfiliz N., Gülmez N., Özcan Z., Yörük M., Erdost H., Aslan Ş., Ergün L., Zık. B., 2009. Veteriner Özel Histoloji, Editör, Özer A. 1. Baskı. Sayfa 73-76, 167-168
  • Gulmez N., Nazli M., Aslan S., Liman N., 2003. Immunolocalisation of serotonin, gastrin, somatostatin and glucagon in entero-endocrine cells of the goose (Anser anser). Acta Vet. Hung., 51, 439-449.
  • Kobayashi S., Fujita T., Sasagawa T., 1971. Electron-microscope studies on the endocrine cells of the humangastric fundus. Arch. Histol. Jap., 32, 429-444.
  • Ku SK., Lee JH., Lee HS., 1998. The histological and immunohistological studies in the Meckel's diverticulum of the duck, Anas platyrhynchos platyrhyncos, Linne. Korean J. Vet. Res., 38, 450-454.
  • Ku SK., Lee HS., Park KD., Lee JH., 2000. Immuno histochemistry of gastrointestinal endocrine cells in the Meckel's diverticulum of the Bean goose, Anser fabalis latham. Korean J. Biol. Sc i., 4, 375 -379.
  • Martinez A., López J., Sesma P., 1993. Development of the diffuse endocrine system in the chicken proventriculus. Cell Tissue Res., 271, 107-113.
  • Naruse H., Gomi T., Kimura A., Adriaensen D., Timmermans JP., 2005. Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells. Anat. Sci. Int., 80, 97-104.
  • Okamoto T., Yamada Y., Iwanaga T., 1980. Distribution and ultrastructure of gastrin cells in the duck digestive tract. Nippon Juigaku Zasshi. 42, 643-649.
  • Oomori Y., Okada Y., Watanabe T., 1997a. Immunohistochemical localization of serotonin, galanin, cholecystokinin and methionine-enkephalin in adrenal medullary cells of the chicken. Tissue Cell, 29, 199-205.
  • Oomori Y., Satoh Y., Ishikawa K., Gesase AP., 1997b. Exocytosis in the antral gastrin cells of mouse, rat, and guinea pig after stimulation by carbamylcholine. Cell Tissue Res., 289, 463–472.
  • Park KD., Lee JH., Ku SK., Lee HS., 1999. An immunohistochemical study on the gastrointestinal endocrine cells in the bean goose, Anser fabalis Latham. Korean J. Vet. Res. 39, 1038-1048.
  • Rawdon BB., 2001. Morphogenesis of gut and distribution of the progenitors of gut endocrine cells at cranial somite levels of the chick embryo. Develop. Dynamics, 222, 153–164.
  • Rawdon BB., Andrew A., 1994. Distribution of serotonin-immunoreactive gut endocrine cells in chicks at hatching. Histochemistry, 102, 93-100.
  • Saito T., Yamada J., Kitamura N., Yamashita T., 1989. An immunohistochemical study on the distribution of endocrine cells in the gastrointestinal tract of domestic pigeon, (Columba livia var domestica). Z. Mikrosk. Anat. Forsc., 103, 237-246.
  • Salvi E., Renda T., 1986. Immunohistochemical studies on the ontogenesis of some endocrine cells in the chicken antrum and duodenum. Basic Appl. Histochem., 30, 307-316.
  • Sikander A., Rana SV., Prasad KK., 2009. Role of serotonin in gastrointestinal motility and irritable bowel syndrome. Clinica Chimica Acta, 403, 47–55.
  • Solcia E., Rindi G., Buffa R., Fiocca R., Capella C., 2000. Gastric endocrine cells: types, function and growth. Regul. Pept., 93, 31-35.
  • Sundler F., Håkanson R., 1991. Gastric endocrine cell typing at the light microscopic level. In: Håkanson R, Sundler F, editor. The stomach as an endocrine organ. Amsterdam: Elsevier, p 9–26
  • Şimşek N., Alabay B., 2008. Light and electron microscopic examinations of the pancreas in quails (Coturnix coturnix japonica). Revue Med. Vet., 159, 198–206.
  • Watanabe T., Chikazawa H., Chungsamarnyart N., Fujioka T., Yamada Y., 1987. Serotonin-storing cells of the chicken duodenum: light, fluorescence, electron microscopy and immunohistochemistry. Cell Tissue Res., 247, 25-32.
  • Wu SV., Wang YH., Campbell BJ., Dimaline R., 1992. Avian progastrin: structure, antral gene expression and biological functions during achlorhydria. Regul. Pept., 40, 283.
  • Yamada J., Sugitani A., Yamashita T., Misu M., 1980. Exocytotic granule release in quail gastrin cells. Biomed. Res., 1, 24-27.
  • Yamaguchi S., Yamada J., Kitamura N., Yamashita T., 1986. Ontogeny of the endocrine cells in the quail proventriculus. Z. Mikrosk. Anat. Forsc., 100, 981-989.
  • Yamaguchi S., Yamada J., Kitamura N., Yamashita T., 1987. Histological and immunohistochemical study on ontogeny of the endocrine cells in the quail gizzard. Gegenbaurs Morpho. Jahrb., 133, 71-78.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makaleleri
Yazarlar

Nejdet Şimşek

Ali Karadeniz Bu kişi benim

Zekeriya Özüdoğru Bu kişi benim

Adem Kara

İsmail Can Bu kişi benim

Yayımlanma Tarihi 21 Aralık 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 6 Sayı: 3

Kaynak Göster

APA Şimşek, N., Karadeniz, A., Özüdoğru, Z., Kara, A., vd. (2011). Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 6(3), 183-193.
AMA Şimşek N, Karadeniz A, Özüdoğru Z, Kara A, Can İ. Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. Aralık 2011;6(3):183-193.
Chicago Şimşek, Nejdet, Ali Karadeniz, Zekeriya Özüdoğru, Adem Kara, ve İsmail Can. “Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin Ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 6, sy. 3 (Aralık 2011): 183-93.
EndNote Şimşek N, Karadeniz A, Özüdoğru Z, Kara A, Can İ (01 Aralık 2011) Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 6 3 183–193.
IEEE N. Şimşek, A. Karadeniz, Z. Özüdoğru, A. Kara, ve İ. Can, “Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 6, sy. 3, ss. 183–193, 2011.
ISNAD Şimşek, Nejdet vd. “Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin Ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 6/3 (Aralık 2011), 183-193.
JAMA Şimşek N, Karadeniz A, Özüdoğru Z, Kara A, Can İ. Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2011;6:183–193.
MLA Şimşek, Nejdet vd. “Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin Ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 6, sy. 3, 2011, ss. 183-9.
Vancouver Şimşek N, Karadeniz A, Özüdoğru Z, Kara A, Can İ. Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2011;6(3):183-9.